| |
| |
| CALCULATING MOLECULAR WEIGHTS OF PEPTIDES |
|
The following table lists amino acids by molecular weight but where one hydroxyl
group (17.0) from the carboxyl end and one hydrogen (1.01) from the amine end
has been removed from the known molecular weight. This allows one to quickly
add up the amino acid weights listed in the table plus any auxilary groups to
determine the calculated molecular weight.
|
| For example: L-ARG-L-PHE-L-ALA acetate.H2O |
| |
ARG: |
156.19 |
| |
| |
PHE: |
147.17 |
| |
| |
ALA: |
71.08 |
| |
| |
acetate: |
60.05 |
| |
| |
water: |
18.01 |
| |
| |
|
|
| |
| |
total: |
452.50 |
| |
| |
|
+18.01 |
* | |
| |
|
|
| |
| calculated formula |
470.51 |
| |
|
| | ALA: |
| 71.08 |
| | ARG: |
| 156.19 |
| | ASN: |
| 114.11 |
|
AUXILARY GROUPS |
|
| | ASP: |
| 115.1 |
|
|
| | CYS: |
| 113.13 |
(Cysteine) |
AC: |
60.05 |
|
| | CYST: |
| 222.30 |
(Cystine) |
H2O: |
18.01 |
|
| | ETH: |
| 145.24 |
|
HIPPURIC: |
162.15 |
|
| | GLU: |
| 129.11 |
|
t-BOC: |
100.22 |
|
| | GLN: |
| 144.11 |
|
CBZ: |
135.14 |
|
| | GLY: |
| 57.05 |
|
PHTHALYL: |
132.11 |
|
| | HIS: |
| 137.14 |
|
TRITYL: |
243.3 |
|
| | HYD-PRO: |
| 113.13 |
|
TOSYL: |
155.18 |
|
| | ILE / LEU: |
| 113.17 |
|
BENZOYL: |
105.10 |
|
| | LYS: |
| 128.17 |
|
CHLOROACETYL: |
77.5 |
|
| | MET: |
| 131.19 |
|
CYCLOHEXYLAMINE: |
99.17 |
|
| | NLE: |
| 113.17 |
|
AMIDE: |
-2.0 |
(subtract) |
| | ORN: |
| 114.14 |
|
HCL: |
36.47 |
|
| | PHE: |
| 147.17 |
|
HBR: |
80.92 |
|
| | PRO: |
| 97.11 |
|
BENZYL: |
89.14 |
|
| | SER: |
| 87.08 |
|
HIPPURYL: |
162.15 |
|
| | THR: |
| 101.10 |
|
NAPHTHYLAMINE: |
143.18 |
|
| | TRP: |
| 186.2 |
|
BENZYLESTER: |
90.12 |
|
| | TYR: |
| 163.17 |
|
BUTYLESTER: |
56.12 |
|
| | VAL: |
| 99.13 |
|
ETHYLESTER: |
28.06 |
|
| | SAR: |
| 71.08 |
|
METHYLESTER: |
14.03 |
|
| | pGLU: |
| 111.10 |
|
SULFUR: |
32.06 |
|
| | cycloLEU: |
| 111.2 |
|
SODIUM: |
22.99 |
|
| |
| * Add 18.01 to final peptide formulas to compensate for hydroxyl group and hydrogen |
| |
|
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