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The Peptide Hormone
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| SECRETIN |
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H-HIS-SER-ASP-GLY-THR-PHE-THR-SER-GLU-LEU-SER-ARG-LEU-ARG-ASP-
SER-ALA-ARG-LEU-GLN-ARG-LEU-LEU-GLN-GLY-LEU-VALAMIDE
Pentaacetate, H2O, synthetic
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Very early this century, in 1902, a biologically active factor which can
stimulate the pancreas to release sodium bicarbonate was isolated in crude
form from hog intestines by Bayliss and Starling1. These
discoverers realizing the messenger role of a chemical substance as a new
concept, coined a new word and called the stimulating factor secretin, a
hormone (derived from the greek word "I move").
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It turned out however, to be rather difficult to achieve its isolation from
gut mucosa in pure form. The pure hormone was finally obtained more than
half a century later by Jorpes and Mutt2 of the Karolinska
Institute in Stockholm in 1961. The same group was also successful in
determining the sequence of the twenty-seven amino acids constituting the
peptide chain of secretin3,4.
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The correctness of the proposed primary structure of porcine secretin,
namely
H-His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg-Asp-Ser-Ala-Arg-
Leu-Gln-Arg-Leu-Leu-Gln-Gly-Leu-Val-NH2,
could subsequently be proved by two synthesis carried out by Bodansky and
his associates5,6,8.
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A new synthesis via a different route was described by Wunsch and his
co-workers9,10,11. Later, on two other synthetic attempts for the
artificial preparation of secretin were published by
Bayerman12,13 and by Geiger14and their respective
collaborators.
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The physiological activity of secretin has been thoroughly
investigated15,16. It was found that in addition to stimulating
pancreatic bicarbonate and biliary secretion, secretin also possesses a
stimulatory action on pancreatic enzyme secretion16, causes in
the stomach19. The cellular localization of secretin by
immunofluorescence techniques in canine duodenum is described by Pearse and
his associates20.
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Investigations in the treatment of duodenal ulcer21,22 were
started in the mid-60's.
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Research Plus offers pure, synthetic
Secretin
for non-human investigations.
This heptacosapeptide does not contain cysteine hcl, a stabilizer. As such,
it can also be used in RIA research. However, without the stabilizer,
secretin must be refrigerated at -20oC in order that its
biological activity is maintained.
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Bibliography:
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Research Plus has developed an extensive bibliography consisting of 466
references, by author, publication and title on the product Secretin as well
as related intestinal hormones. It is free for the
asking.
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References:
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| 1. |
W.M.Bayliss and E.H.Starling, Proc.Roy.Soc. 69, 352
(1902) |
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| 2. |
J.E.Jorpes and V.Mutt, Acta Chim.Scand. 15, 1790
(1961) |
| 3. |
V.Mutt, S.Magnusson, J.E.Jorpes and E.Dahl,
Biochemistry 4,2358 (1965) |
| 4. |
V.Mutt, J.E.Jorpes and S.Magnusson,
Eur.J.Biochem. 15, 513 (1970) |
| 5. |
M.Bodansky, M.A.Ondetti, S.D.Levine, V.L.Narayanan,
M.VonSaltza, J.T.Sheehan,N.J.Williams and E.P.Sabo, Chem.Ind.(London)
1757 (1966) |
| 6. |
M.Bodansky and N.J.Williams, J.A.C.S., 89,685
(1967)
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| 7. |
M.Bodansky, M.A.Ondetti, S.D.Levine and
N.J.Williams, J.A.C.S., 89,6753 (1967) |
| 8. |
M.A.Ondetti, V.L.Narayanan, M.VonSaltza,
J.T.Sheehan, E.F.Sabo and M.Bodansky, J.A.C.S., 90,4711 (1968) |
| 9. |
W.Wunsch, Naturwissenschaften 59,239 (1972) |
| 10. |
E.Wunsch and G.Wendlberger, Chem.Ber., 105,2508
(1972) |
| 11. |
E.Wunsch, E.Jaeger, M.Deffner, R.Scharf and
P.Lehnert, Chem.Ber., 105, 2515 (1972) |
| 12. |
A.Van Zon and H.C.Beyerman in "Peptides 1974",
Proceedings of the 135th Europ. Peptide Symposium,Ed.Y.Wolman,
John Wiley & Sons, N.Y., Israel U.Press.Jer.S. 165 |
| 13. |
A.Van Zon, Thesis, Technische Hogeschool Delft,
Netherlands, Delftse Universitaire Pers. (1974)
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| 14. |
G.Jaeger, W.Konig, H.Wissman and E.Geiger,
Chem.Ber. 107, 215 (1974) |
| 15. |
W.M.Bayliss and E.H.Starling, J.Physiol, 28, 325
(1902b)
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| 16. |
For a review see: J.E.Jorpes and V.Mutt (Eds)
"Secretin, Cholecystokinin, Pancreozymin and Gastrin", Springer-Verlag,
Berlin, Heidelberg, N.Y. (1973*)
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| 17. |
J.Dupre et al., a) Lancet 11, 26 (1966); b) Canad.
Med. Assoc. J. 94, 663 (1966) |
| 18. |
H.Stahlheber, M.Reiser, P.Lehnert, M.M.Forell,
P.Botterman and E.Jaeger, Klin. Wochenschrift 53, 339 (1975) |
| 19. |
K.G.Wormsley and M.I.Grossman, Gastroenterology 47,
72 (1964) |
| 20. |
J.M.Polak, S.Bloom, I.Crulling and A.G.E.Pearse,
Gut 12, 605 (1971) |
| 21. |
L.Demling, R.Ottenjann and H.Gebhardt,
Gastroenterologia 102, 129 (1964)
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| 22. |
M.I.Gorssman, Gastroenterology 50, 912 (1966) |
| * For another review see: M.I.Grossman,
"Gastrointestinal Hormones; a panoramic view":a lecture delivered to the
Endocrine Society, New York. |
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| 19-2708-02
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SECRETIN, PENTAACETATE, Porcine, Synthetic
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| 19-2772-02
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SECRETIN, HEXAACETATE, Porcine, Synthetic
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(His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg-
Asp-Ser-Ala-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Gly-Leu-Val-NH2.
acetate)
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m.w. 3055.46 (free peptide)
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19-2773-02
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SECRETIN, HEXAASPARATATE, Porcine, Synthetic
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(His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg-
Asp-Ser-Ala-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Gly-Leu-Val-NH2.
hexxaaspartate)
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m.w. 3055.46 (free peptide)
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19-2780-02
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SECRETIN, Human, Synthetic
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(His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg-Glu-Gly-Ala-Arg-
Leu-Leu-Gln-Gly-Leu-Val-NH2.acetate)
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m.w. 3039.5 (C130H220N44
O40)
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